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CD spectra of 2,4-dihydroxy-2-hydroxymethyl-3-oxo-butanoic acid produced by the PDHc-E1 E636A and E636Q variants. (a) PDHc-E1 E636A + β-hydroxypyruvate and (b) PDHc-E1 E636Q + β-hydroxypyruvate. (λmax= 301 nm). Conditions are described under Materials and methods.
