Table 1.
Enantiomeric excess of acetoin, phenylacetylcarbinol, and acetolactate formed by the various enzymes used and percent yields for the products formed under the specified conditions.
| Enzyme variant | enantiomeric excess and yield of product a |
||
|---|---|---|---|
| PAC | Acetoin | Acetolactate | |
| YPDC-E477Q | >99%(R)b (62) | 94% (R)b (98) | not produced |
| YPDC-D28A | >99% (R)b (60) | 88% (R)b (68) | 40% (S)c (92) |
| PDHc-E1 E636A | not produced | 24% (S)b (92) | 60% (R)c (93) |
| PDHc-E1 E636Q | not produced | 70% (S)b (93) | >99% (R)c (95) |
a
Yield in parenthesis in %.
b
ee was calculated by Chiral-GC.
c
ee was calculated from CD data.