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. 2006 Nov 27;34(22):6404–6415. doi: 10.1093/nar/gkl727

Figure 2.

Figure 2

Determination of steady-state levels of tRNALeu(UUR) in cybrid clones. (A) Levels of tRNAs analysed by high-resolution PAGE followed by blot hybridization in two control cell lines and the cybrid clone containing 77% of the 3302A>G mutation and 98% of the 3302A>G mutation, as well as a clone containing the 3243A>G mutation (3243 100%). RNA was loaded in two concentrations in order to ensure linearity of the densitometric signal. (B) The densitometric values for 1 μg of 143B RNA were arbitrarily set to 1.0 in order to get an estimate of the variability of the measurements. Upper panel: levels of mt-tRNALeu(UUR) (black bars) and tRNAVal (grey bars) were normalized to nuclear encoded 5S rRNA; lower panel: Levels of mt-tRNALeu(UUR) were normalized to tRNAVal.