Skip to main content
. 2006 Nov 27;34(22):6404–6415. doi: 10.1093/nar/gkl727

Figure 6.

Figure 6

Determination of the half-life of mitochondrial tRNAs in cybrid clones treated with EtBr for up to 48 h. (A) Decay of mitochondrial tRNAs was analysed by high-resolution PAGE followed by blot hybridization in 143B control cells, in the cybrid clone containing 98% of the 3302A>G mutation, as well as in the clone containing the 3243A>G mutation (3243 100%). RNA (0.5 μg) was loaded in triplicate per sample in order to minimize data acquisition errors of the signals. (B) Densitometric data for tRNALeu(UUR), tRNAVal and tRNALys were normalized to nuclear encoded 5S rRNA.