Figure 6.

Incorporation of cytidine analogues with HeLa nuclear extract. The transcripts were analyzed by denaturing 5% polyacrylamide gel electrophoresis. Above each lane, rNTP added to the extract are recorded. Molar ratios of ATP:(³²P-)GTP:CTP:UTP:C*TP is 1:0.04:1:1:1 if present (A). Two-dimensional cellulose TLC for nearest-neighbor analyses of the transcripts (B). In the transcription, the CTP analogues, ho⁴CTP, mo⁴CTP and rPTP, respectively, were added in place of UTP, while sc⁴CTP was added in place of CTP.