Table 1.
Steady-state kinetics data for insertion opposite cytidine analogues, ho4C, mo4C, rP and sc4C in RNA templates by HIV RT
| T:P | Km (μM) | Vmax,rel (%/s) (× 10−2) | Vmax,rel/Km | fins |
|---|---|---|---|---|
| U:dA | 2.5 ± 0.5 | 15 ± 2 | 6.3(± 1.4) × 10−2 | 1 |
| U:dG | 59 ± 17 | 3.1 ± 0.3 | 5.4(± 1.0) × 10−4 | 8.8(± 1.6) × 10−3 |
| U:dC | 2038 ± 575 | 6.0 ± 1.3 | 3.1(± 1.0) × 10−5 | 5.0(± 1.3) × 10−4 |
| U:dT | 2680 ± 381 | 1.8 ± 0.4 | 6.8(± 0.7) × 10−6 | 1.1(± 0.2) × 10−4 |
| ho4C:dA | 70 ± 13 | 22 ± 1 | 3.2(± 0.6) × 10−3 | 5.1(± 0.4) × 10−2 |
| ho4C:dG | 62 ± 4 | 11 ± 2 | 1.7(± 0.4) × 10−3 | 2.8(± 0.5) × 10−2 |
| ho4C:dC | 1963 ± 1086 | 7.8 ± 1.7 | 4.5(± 1.4) × 10−5 | 7.5(± 3.0) × 10−4 |
| ho4C:dT | 2239 ± 807 | 11 ± 1 | 5.5(± 2.9) × 10−5 | 8.7(± 3.9) × 10−4 |
| mo4C:dA | 131 ± 15 | 20 ± 2 | 1.5(± 0.2) × 10−3 | 2.6(± 1.0) × 10−2 |
| mo4C:dG | 158 ± 75 | 9.8 ± 1.7 | 6.9(± 2.5) × 10−4 | 1.1(± 0.3) × 10−2 |
| mo4C:dC | 2742 ± 962 | 3.7 ± 0.8 | 1.5(± 0.8) × 10−5 | 2.3(± 0.9) × 10−4 |
| mo4C:dT | 3486 ± 477 | 5.8 ± 0.3 | 1.7(± 0.3) × 10−5 | 2.8(± 0.6) × 10−4 |
| rP:dA | 2.4 ± 0.2 | 26 ± 7 | 1.1(± 0.3) × 10−1 | 1.9 ± 0.7 |
| rP:dG | 15 ± 2 | 14 ± 2 | 9.7(± 3.2) × 10−3 | 1.6(± 0.6) × 10−1 |
| rP:dC | 1870 ± 296 | 7.6 ± 0.9 | 4.2(± 1.2) × 10−5 | 7.0(± 2.5) × 10−4 |
| rP:dT | 1730 ± 457 | 3.7 ± 0.3 | 2.3(± 0.8) × 10−5 | 3.7(± 1.2) × 10−4 |
| sc4C:dA | 234 ± 21 | 5.3 ± 1.3 | 2.3(± 0.7) × 10−4 | 3.9(± 2.0) × 10−3 |
| sc4C:dG | 5.0 ± 0.7 | 3.1 ± 0.0 | 6.3(± 0.8) × 10−3 | 1.0(± 0.3) × 10−1 |
| C:dG | 1.7 ± 0.9 | 6.0 ± 0.8 | 3.8(± 1.2) × 10−2 |
No extended products from sc4C:dC and sc4C:dT.