Table 2.
Steady-state kinetics data for extension from dA or dG paired with a cytidine analogue, ho4C, mo4C, rP or sc4C by HIV RT
| T:P | Km (μM) | Vmax,rel (%/s) (×10−2) | Vmax,rel/Km | fext |
|---|---|---|---|---|
| U:dA | 0.17 ± 0.04 | 4.3 ± 0.2 | 2.6(± 0.4) × 10−1 | 1 |
| ho4C:dA | 2.6 ± 0.2 | 8.5 ± 0.1 | 3.3(± 0.2) × 10−2 | 1.3(± 0.3) × 10−1 |
| mo4C:dA | 2.9 ± 1.1 | 10 ± 2 | 3.7(± 0.8) × 10−2 | 1.5(± 0.5) × 10−1 |
| rP:dA | 0.18 ± 0.04 | 6.7 ± 0.1 | 3.8(± 0.7) × 10−1 | 1.5 ± 0.5 |
| sc4C:dA | 4.6 ± 1.8 | 1.5 ± 0.2 | 3.5(± 1.2) × 10−3 | 1.3(± 0.3) × 10−2 |
| U:dG | 1600 ± 494 | 14 ± 3 | 9.2(± 1.9) × 10−5 | 3.6(± 0.9) × 10−4 |
| ho4C:dG | 166 ± 7 | 17 ± 1 | 1.0(± 0.1) × 10−3 | 3.8(± 0.8) × 10−3 |
| mo4C:dG | 162 ± 29 | 18 ± 1 | 1.1(± 0.1) × 10−3 | 4.3(± 0.8) × 10−3 |
| rP:dG | 149 ± 6 | 15 ± 1 | 1.0(± 0.1) × 10−3 | 3.9(± 1.0) × 10−3 |
| sc4C:dG | 48 ± 15 | 2.6 ± 0.04 | 5.9(± 2.1) × 10−4 | 2.4(± 1.3) × 10−3 |
| C:dG | 72 ± 14 | 10 ± 1 | 1.5(± 0.4) × 10−3 |