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. 2006 Nov 27;34(22):6472–6487. doi: 10.1093/nar/gkl861

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© 2006 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Influence of NFI over-expression on p21 promoter activity in growth-arrested HCT116 cells. (A) Crude nuclear extracts were prepared from both HCT116 and HeLa cells (used as a control) and examined for expression of Sp1 and NFI in Western blot analyses. (B and C) The p21–192 recombinant construct was co-transfected with the empty vector pCH (+EV), or with expression plasmids encoding each of the NFI isoforms, either individually (+NFI-A, -B, -C and -X) or in combination (+NFI-BCX), in doxorubicin, growth-arrested HCT116 cells. Cells were collected 48 h later and used either for the measurement of the CAT activity (panel B) or to determine the proportion of the cells engaged in the S-phase of the cell cycle by FACS analyses (panel C).