Figure 3.

Cleavage of A/C mismatch in synthetic K-ras G13D sequence by Y80A Tma endonuclease V mutant. Cleavage reactions were performed as described in Materials and Methods with 2.5 mM MnCl₂. (A) Schematic illustration of A/C cleavage. A/C heteroduplex was formed by annealing of 5′-FAM-TAACTTGTGGTAG TTGGAGCTGGTGACGTAGGCAAGAGTGCCTTGACGATACAGCTAATTCATTCC-3′ and 5′-TET-TGAATTAGCTGTAGCGTCAAGGCACTCTTGCCTACGCCACCA GCTCCAACTACCACAAGT-3′. C/A heteroduplex was formed by annealing of 5′-FAM-TATCGTCAAGGCACTCTTGCCTA CGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTCC-3′ and 5′-TET-TGACTGATAATAAA CTTGTGGTAGTTGGAGCTGGTGACGTAGGCAAGAGTGCCTTGACGA-3′. (B) Cleavage of A/C K-ras G13D mismatch by wild-type Tma endo V and Y80A mutant.