Fig. 2.

Localization of dysferlin in brains of nondemented subjects. a, b Demonstrates the localization of dysferlin immunoreactivity in the brain. There is a neuronal expression of dysferlin localized in cytoplasm of large pyramidal (layers III, V, VI) neurons in the frontal cortex at low power (a). Little to no neuritic staining was detected. Dysferlin expression is confined to neurons (arrow, DAB brown in b) and is not seen in glial cells stained with GFAP (small arrowheads, Vector Red pink in b). Magnification of (a) is 100× and the scale bar in (a) = 50 μm. Magnification of (b) is 400× and the scale bar in (b) = 20 μm