Fig. 1.

Expression levels of rpb4 mRNA when transcribed from different promoter constructs. Cells expressing rpb4 from the P3nmt1, P41nmt1 or P81nmt1 promoter were grown for 24 h in EMM medium at 32°C (promoter on; gray bars) or cultivated for a further 21 h in the presence of thiamine (promoter off; white bars). rpb4 (left) and fba1 (right; control) mRNA levels from the same cells were measured by quantitative RT-PCR. The PCR was performed on reverse transcription reactions, which were carried out in the presence (RT+) or absence (RT−) of reverse transcriptase