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. 2007 Jan;13(1):15–21. doi: 10.1261/rna.228707

FIGURE 1.

FIGURE 1.

Editing of different aa-tRNAs by different aaRSs or editing domains. Deacylation of [3H]Leu-tRNALeu (•), [3H]Ile-tRNALeu (○), [3H]Ile-tRNAIle (▾), [3H]Val-tRNAIle (▿), [3H]Val-tRNAVal (▪), and [3H]Thr-tRNAVal (□) by (A) 5 μM BsValRS, (B) 5 μM BsVal-CP1, (C) 5 μM EcIleRS, (D) 5 μM EcIle-CP1, (E) 5 μM AaLeuRS (50 nM AaLeuRS in the inset), and (F) 5 μM AaLeu-CP1. tRNAs originated from (A–D) E. coli or (E,F) A. aeolicus. The background hydrolysis of aminoacyl-tRNA in the absence of enzyme (<15% over 10 min and 30% for Leu-tRNALeu) was subtracted from these plots for normalization purposes. Values were obtained from three independent determinations.