FIGURE 2.

Graphical representation of the k _obs for the cleavage reaction of the Swt (open circles) and the Ss⁴U-2 (closed squares) substrates as a function of the ribozyme concentration. The inset shows an autoradiogram of a denaturing PAGE gel of the cleavage reactions performed with the Ss⁴U-2 substrate and a ribozyme concentration of 200 nM. The positions of the bromophenol blue (BPB), the substrate (S), and the product (P) are indicated. From left to right, the aliquots were recovered after 2, 5, 10, 15, 20, and 40 min of reaction.