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. Author manuscript; available in PMC: 2006 Dec 19.
Published in final edited form as: J Biol Chem. 2004 Mar 11;279(20):20663–20671. doi: 10.1074/jbc.M400272200

Fig. 5.

Fig. 5

The starvation-induced processing of Ape1p was inhibited by Ras/PKA signaling activity. The CUP1-APE1 construct was introduced into strains with the indicated genotypes. The strains were grown to mid-log phase at 30 °C in a YM-glucose medium containing 100 μm CuSO4 to induce high level expression from the CUP1 promoter. The cultures were then transferred to the nitrogen starvation medium, SD-N, and incubated at 30°C for 3, 7, or 15 h. Protein extracts were prepared from these cultures, and the relative levels of Ape1p processing were assessed with a Western immunoblot using a polyclonal antiserum specific for Ape1p. The relative processing at each starvation point (S) was compared with that observed in the mid-log phase, or nonstarved, control culture (N). The positions of the mature and precursor forms of Ape1p are shown. The strains analyzed were wild-type (TN125), RAS2val19 (PHY3513), atg1Δ (YYK126), and atg13Δ (YYK130). All strains except PHY3513 also carried the control vector, pRS413.