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. Author manuscript; available in PMC: 2006 Dec 19.
Published in final edited form as: J Biol Chem. 2004 Mar 11;279(20):20663–20671. doi: 10.1074/jbc.M400272200

Fig. 6.

Fig. 6

The rapamycin-induced dephosphorylation of Atg13p was not inhibited by the presence of the RAS2val19 allele. Wild-type and RAS2val19 cells carrying a high-copy ATG13 plasmid were grown to mid-log phase and then treated with 0.2 μg/ml rapamycin for 0 or 60 min. Protein extracts were prepared and the relative mobility of Atg13p in an SDS-polyacrylamide gel was assessed by Western immunoblotting with a polyclonal antiserum specific for Atg13p. The positions of the hyperphosphorylated forms of Atg13p found in log phase cells are indicated (pp-Atg13p). The strains analyzed were wild-type (TN125 with the control vector, pRS413) and RAS2val19 (PHY3513). The strains were grown in either medium lacking methionine (wild-type and RAS2val19 (on)) or containing 500 μm methionine (RAS2val19 (off)).