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. 2003 Jul 28;100(16):9138–9143. doi: 10.1073/pnas.1233634100

Fig. 3.

Fig. 3.

(A) Extents of expression in COS-7 cell cultures of the β-gal gene mediated in the presence of serum by PEI2 (bars a–c) and PEI2–GNPII (bars d–f) at different N/P ratios. Bars a and d, N/P = 90; bars b and e, N/P = 120; bars c and f, N/P = 150. (B) Extents of expression in COS-7 cell cultures of the β-gal gene mediated in the presence of serum by PEI25 (bar a), dodecyl–PEI2 (bars b and c), PEI2–GNPII (bar d), and PEI2–GNPII + dodecyl–PEI2 (bars e and f). Bar a, N/P = 10; bar b, N/P = 20; bar c, N/P = 40; bar d, N/P = 150; bar e, N/P = 150 + 20; bar f, N/P = 150 + 40. Cells transfected with the plasmid in the absence of polycations showed no appreciable β-gal activity.