Table 1. Ectopic expression of Drosophila Orc6 in L2 cells.
Drosophila Orc6 protein | BrdUrd incorporation, % nuclei | Multinucleated cells, % cells |
---|---|---|
Orc6-WT | 70-80 | 0-0.2 |
Orc6-224 | 70-80 | 5-7 |
Orc6-200 | 70-80 | 25-30 |
Orc6-163 | 2-5 | ND* |
Control† | 70-80 | 0-0.2 |
Not determined; abnormal cell morphology obscured nuclei.
No transfection control; cells have endogenous ORC.