Fig. 3.

Northern blot analysis of B. mori pgFAR. (A) Expression in adult tissues. Lanes: 1, head; 2, fat body; 3, Malpighian tubule; 4, flight muscle; 5, ovary; 6, testis; 7, pheromone gland. Each tissue was dissected from a newly emerged female moth (day 0). Testis was dissected from a newly emerged male moth. (B) Expression in the pheromone gland. Pheromone glands dissected from females before and after adult eclosion were used for total RNA extraction. Day 0 corresponds to the day of eclosion. Ribosomal RNA band stained with ethidium bromide is shown as a loading control. The heterogeneity of the transcripts is caused by the differences in their 3′-untranslated regions (data not shown).