SK–Pg interactions. (A) A ribbon diagram illustrates the
crystal structure of human Pg. The loop structures that contact the α
and γ domains of SK are numbered and colored. This figure was produced
by using molscript and raster3d
(36,
37). (B and
C) Activation of cow Pg, human micro-Pg, and chimeric micro-Pgs by
SUPA (B) and SK (C). Human micro-Pg (300 nM), cow Pg (300
nM), and chimeric loop 5, 7, 9, 11 micro-Pg (300 nM) (or other chimeric
micro-Pgs) were activated by SK (20 nM) or SUPA, as indicated. Pg activation
was monitored as described in Fig.
1. (Inset) Activation of the loop 5, 7, 9, and 11
chimeric micro-Pg (300 nM) by urokinase (33 nM).