Fig. 4.

Analysis of polyserase-I expression in human tissues and tumor cell lines. Northern blot analysis of polyserase-I expression in fetal and adult human tissues and a panel of tumor cell lines. Approximately 2 μg of polyadenylated RNA from the indicated tissues or tumor cell lines was hybridized with a 5′ probe of the polyserase-I full-length cDNA. The positions of the RNA markers are shown. The filters were subsequently hybridized with an actin probe to ascertain the differences in RNA loading among the different tissues.