Fig. 2.

Basal activity of the mutantαβ^Cys-105 enzyme is comparable to the activity of NO-treated sGC. Specific activity of the purified mutant (filled bars) and wild-type (open bars) enzymes in the absence (basal) or presence of 100 μM SNP was determined. Data are shown as means ± SD of three independent experiments performed in triplicate. (Left Inset) UV-Vis spectra of 3 μM purified wild-type and mutant sGC. AU, arbitrary units. (Right) Coomassie blue staining of 5 μg of the wild-type (W) and mutant (M) sGC preparation. The size (in kilodaltons) of the molecular mass markers (PM) is indicated.