Table 1. Activity of mutant sGC in intact Sf9 cells or neuroblastoma cell lysates.

	Sf9 with mutant*		Sf9 with wild type*		BE2/pCDNA3.1 βCys-105		BE2/pCDNA3.1 vector
	Basal	NO†	Basal	NO†	Basal	NO†	Basal	NO†
cGMP levels, nmol/mg of protein	35.0 ± 1.59	35.8 ± 1.42	0.4 ± 0.02	4.9 ± 0.13
sGC activity, pmol/min per mg of protein					60.0 ± 12.1	150.2 ± 8.2	<5‡	300.4 ± 15.9

Values are means ± SD of three measurements.

^*cGMP levels 48 h postinfection for αβ^Cys-105 and 72 h postinfection for αβ sGC.

^†250 μM NO donor spermine NONOate was used for intact Sf9 cells and 100 μM SNP was used for BE2 lysates.

^‡The activity is at or lower than the detection level for this method.