Table 2. Absorption spectra of various forms of native and altered hemoproteins.
| n.u.v. | Soret | β | α | Ref. | |
|---|---|---|---|---|---|
| Wild-type sGC | |||||
| Ferric | 393 | 14, 27 | |||
| Ferrous (native) | 431 | 560-590 | 560-590 | 27 | |
| Ferrous + CO | 423 | 541 | 567 | 14 | |
| Ferrous + NO | 399 | 537 | 572 | 14, 27 | |
| Reconstituted αβCys-105 | sGC | ||||
| Ferric | 365 | 417 | 542 | 573 | This study |
| Ferrous | ND | 426 | 540 | This study | |
| Ferrous + CO | ND | 421 | 536 | 567 | This study |
| Ferrous + NO | 399 | 567 | 611 | This study | |
| Cytochrome P450 | |||||
| Ferric | 393 | 645 | 39, 40 | ||
| Ferrous | 411 | 542 | 39, 40 | ||
| Ferrous + CO | 366 | 447 | 39, 40 | ||
| Cytochrome P420 | |||||
| Ferric | 366 | 418 | 540 | 570 | 39, 40 |
| Ferrous | 325 | 428 | 535 | 562 | 39, 40 |
| Ferrous + CO | 341 | 423 | 541 | 570 | 39, 40 |
Values for the αβCys-105 mutant are reported. n.u.v., near-UV band; ND, not detected because of the overlapping dithionate absorption but absent when DTT was used (data not shown).