Table 3. Analysis of the total protein list with respect to functional categories.
Proteins detected
|
Membrane proteins
detected*
|
mRNA detected
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Category | Genes/proteins | Membrane proteins* | Mean no. of tryptic peptides predicted† | Mean mRNA signal (arithmetic)† | Mean mRNA signal (geometric)† | No. | % | No. | % | No. | % |
1 Cell processes | 608 | 355 | 12.6 | 2,000 | 550 | 171 | 28.1 | 63 | 17.7 | 431 | 70.9 |
2 Extrachromosomal | 92 | 12 | 14.2 | 1,270‡ | 210‡ | 8 | 8.8 | 4 | 33.3 | 25 | 48.1‡ |
3 Global functions | 55 | 12 | 15.8 | 2,610 | 1,050 | 22 | 40.7 | 3 | 25.0 | 47 | 85.5 |
4 Macromolecule metabolism | 315 | 43 | 19.4 | 3,100 | 1,090 | 156 | 49.7 | 15 | 34.9 | 283 | 89.8 |
5 Metabolism of small molecules | 881 | 111 | 15.6 | 2,630 | 820 | 385 | 43.7 | 25 | 22.5 | 673 | 76.4 |
6 Miscellaneous | 742 | 180 | 14.1 | 780§ | 280§ | 117 | 15.8 | 14 | 7.8 | 421 | 57.4§ |
7 Open reading frames¶ | 1,409 | 258 | 9.8 | 780∥ | 280∥ | 199 | 14.1 | 18 | 7.0 | 803 | 57.7∥ |
8 Structural elements | 215 | 46 | 11.6 | 17,000 | 2,160 | 89 | 46.8** | 18 | 39.1 | 1,369 | 78.6 |
9 tRNA | 86 | — | — | 7,800 | 2,150 | — | — | — | — | 71 | 82.6 |
10 Sum or average†† | 4,403 | 1,017 | 13.1 | 2,470‡‡ | 490‡‡ | 1,147 | 26.7** | 160 | 15.7 | 2,923 | 67.4‡‡ |
High estimate.
Data are for all proteins/genes in category. The values for arithmetic means for expressed genes in each category were as follows: 1, 2,770; 2, 2,530; 3, 3,020; 4, 3,420; 5, 3,400; 6, 1,260; 7, 1,260; 8, 21,590; 9, 9,430; 10, 3,600. The values for geometric means for expressed genes were as follows: 1, 1,060; 2, 830; 3, 1,530; 4, 1,360; 5, 1,470; 6, 620; 7, 680; 8, 5,000; 9, 4,810; 10, 1,090.
Forty genes were not represented on Affymetrix GeneChips.
Nine genes were not represented on Affymetrix GeneChips.
Seventeen genes were not represented on Affymetrix GeneChips.
Stable RNAs were excluded from calculations.
As appropriate.
Sixty-six genes were not represented on Affymetrix GeneChips.