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. 2003 Jul 22;100(16):9250–9255. doi: 10.1073/pnas.1133325100

Fig. 1.

Fig. 1.

Effect of EcoSSB on N4 vRNAP runoff transcription of the ssDNA template 12-P2–52. (A) Where indicated, EcoSSB and vRNAP were present at 1 μM and 10 nM, respectively. (B) A 100-μl complete transcription mixture containing 1 nM template but no EcoSSB was divided into four aliquots (I, II, III, and IV) before incubation at 37°C. I, no additions; II, 50 nM template was added after 20 min incubation; III, 1 μM EcoSSB was added before incubation; and IV, 1 μM EcoSSB was added after 20 min of incubation. At the indicated times, 5-μl aliquots were analyzed.