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. 2003 Jul 22;100(16):9250–9255. doi: 10.1073/pnas.1133325100

Fig. 2.

Fig. 2.

Effect of EcoSSB on the state of the DNA template and the RNA product. (A and B) Transcription reactions contained 5 nM unlabeled 12-P2–52 DNA template and [α-32P]UTP. (C and D) Transcription reactions contained 5 nM 5′ 32P-labeled template and unlabeled NTPs. Where indicated, EcoSSB was present at 1 μM. After a 15-min incubation, each mixture was divided into three aliquots and treated for 15 min at 37°C with no additions, RNase H, or Nuclease S1, as indicated, and analyzed by native PAGE.