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. 2003 Jul 22;100(16):9250–9255. doi: 10.1073/pnas.1133325100

Fig. 5.

Fig. 5.

Mutant and chimeric SSB proteins and their effect on vRNAP transcription. (A) Schematic representation of mutant and chimeric proteins (12). DNA-binding domains (striped); EcoSSB C-terminal 10-aa region (filled); linker sequence between Eco SSB DNA-binding domain and the C-terminal 10-aa region (open); and Hsmt SSB unique N-terminal and C-terminal sequences (shaded). (B) The effect of mutant and chimeric SSB proteins on vRNAP transcription. Symbols are presented to the right of schematic representation in A. (C) Sequence of the C-terminal 13 aa of the EcoSSB (7), P1 SSB (13), and F SSB (33). (D) Effect of P1 SSB and F SSB on vRNAP transcription. Reaction mixtures containing no SSB or the indicated concentrations of different SSB proteins, 3 nM 12-P2–52 DNA template and 1 μM mini-vRNAP were incubated for 5 min at 37°C, and the products were analyzed on 8 M urea/8% polyacrylamide gels.