Table 1. RNA aptamer affinities for different forms of murine NF-κB.

Construct	(p50)2*	p50/p65†	(p65)2‡	p50dd/p65§	p50/p65dd¶	p50dd/p65dd∥
Kd ± SD**	5.4 ± 2.2	22 ± 7	3,500 ± 2,300	187 ± 115	10 ± 4	NB††

Equilibrium dissociation constant estimates (nM) were obtained by nitrocellulose filter binding as described in Methods.

^*Homodimer of p50 RHR (amino acids 39-363).

^†Heterodimer of p50 RHR (amino acids 37-363) and p65 RHR (amino acids 19-291).

^‡Homodimer of p65 RHR (amino acids 1-325).

^§Heterodimer of p50 dimerization domain (amino acids 245-350) and p65 RHR (amino acids 19-304).

^¶Heterodimer of p65 dimerization domain (amino acids 191-304) and p50 RHR (amino acids 39-350).

^∥Heterodimer of p50 dimerization domain (amino acids 245-350) and p65 dimerization domain (amino acids 191-304).

^**Mean ± SD based on at least two determinations.

^††NB, no binding detected.