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. 2003 Jul 25;100(16):9280–9285. doi: 10.1073/pnas.0832269100

Fig. 4.

Fig. 4.

Tracking the transport and acidification of individual influenza viruses. (a) The trajectory of a Cy3/CypHer5-labeled virus inside a cell. The colored bar indicates a uniform time axis from 0 to 450 s. The green star indicates the initial acidification site. (Scale bar: 10 μm.) (b) Time trajectories of the velocity (black) and the ratio of CypHer5 and Cy3 fluorescent emission (red) of the virus. t4 defines the time elapsed between the end of stage II and the initial acidification event. The pH values are labeled according to the calibrated pH dependence of the intensity ratio between CypHer 5 and Cy3 (data not shown). (c) Histograms of t3 and t4 as defined in Figs. 2b and 4b, respectively.