Fig. 1.
The murine locus encodes BADL and BADS. Alternative transcription initiation and translation start sites result in 28-kDa BADL and 23-kDa BADS. (A) Alternative first exons result in two Bad mRNA species. (B) Northern blot analysis with a Bad cDNA probe confirms two mRNA species in which the 1.5-kb mRNA contains exon 1a (Ex1a). (C) In vitro coupled transcription/translation indicates that exon 1a containing mRNA results in BADL and BADS, whereas exon 1b containing mRNA results in BADS. (D) Western blot analysis of BADL and BADS.