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. 2003 Jul 16;100(16):9342–9347. doi: 10.1073/pnas.1633516100

Fig. 4.

Fig. 4.

Effect of GRIM-19 on STAT3 activation and DNA binding. (A–C) MCF-7 cells stably transfected with empty vector (V) or vector expressing myc-tagged GRIM-19 (G) were stimulated with IL-6 (10 ng/ml). Cell lysates were analyzed by the Western method (WB) with antibodies specific for native or phospho-STAT3. (D) Nuclear extracts (5 μg) from IL-6-stimulated cells were used for EMSA with a STAT3 binding element. N, no extract. (E–G) Total nuclear protein (70 μg) was used in Western analyses. (H) The effect of recombinant GRIM-19 on DNA binding of STAT3. Reactions were incubated with buffer (B) or 4 μg of recombinant GRIM-19 (G). Lanes 1 and 2, no nuclear extract; lanes 3 and 4, nuclear extract from IL-6-treated cells.