Fig. 2.

MIS regulates endogenous FSHβ mRNA levels. Total RNA was prepared from LβT2 cells treated with 35 nM MIS for the indicated times. Equal amounts of RNA were subjected to combined reverse transcription and QRT-PCR with primers specifictoFSHβ (A) and LHβ (B). The concentration threshold (Ct) values of at least three repeats were averaged, and differences from control were plotted after normalizing a Ct value difference of 1- to 2-fold. Values represent mean ± SEM. * and ** represent significant differences P < 0.05 and P < 0.001, respectively, compared with untreated (NT) controls.