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. 2003 Jul 23;100(16):9354–9359. doi: 10.1073/pnas.1533295100

Fig. 2.

Fig. 2.

Validation of the MIF-KO mouse. (A) EcoRI-digested genomic DNA prepared from WT (+/+), heterozygous (+/–), and MIF-KO (–/–) mice was hybridized with the external 5′ probe. (B) Northern blot analysis of RNA prepared from the spleens of WT (+/+), heterozygous (+/–), and MIF-KO mice (–/–) using MIF cDNA as probe. (C) Immunoblot analysis of protein extracts prepared from the livers of WT (+/+), heterozygous (+/–), and MIF-KO mice (–/–) using a MIF-specific polyclonal antibody (R102). The expression of mitogen-activated protein kinase ERK1/2 is shown as a loading control. rMIF, recombinant MIF.