Validation of the MIF-KO mouse. (A) EcoRI-digested
genomic DNA prepared from WT (+/+), heterozygous (+/–), and MIF-KO
(–/–) mice was hybridized with the external 5′ probe.
(B) Northern blot analysis of RNA prepared from the spleens of WT
(+/+), heterozygous (+/–), and MIF-KO mice (–/–) using
MIF cDNA as probe. (C) Immunoblot analysis of protein
extracts prepared from the livers of WT (+/+), heterozygous (+/–), and
MIF-KO mice (–/–) using a MIF-specific polyclonal antibody (R102).
The expression of mitogen-activated protein kinase ERK1/2 is shown as a
loading control. rMIF, recombinant MIF.