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Growth characterization of MIF–/–p53–/– DKO fibroblasts. (A) The deletion of the Trp53 gene in DKO mice was confirmed by PCR amplification, using the primers specific for the p53 WT and KO (neo) allele. (B) Representative growth curves of p53–/–, DKO, and MIF-KO fibroblasts. (C) Immunoblot analysis of proliferating (P) and confluent (C) DKO and p53–/– fibroblasts with the indicated antibodies. The expression of p21 by MIF-KO MEFs (Right) is shown as a control.
