Table 2. ntl and tbx6 act competitively to regulate MyoD expression in adaxial cells.
|
RNA injected, pg
|
MyoD expression pattern, %
|
|||
|---|---|---|---|---|
| ntl | tbx6 | Suppressed | WT | Ectopic |
| 50 | — | 0 | 7 (2/30) | 93 (28/30) |
| — | 200 | 90 (17/19) | 11 (2/19) | 0 |
| 50 | 200 | 48 (15/31) | 48 (15/31) | 3 (1/31) |
| 100 | 200 | 31 (10/32) | 56 (18/32) | 13 (4/32) |
Pattern of MyoD expression in embryos with ectopically expressed No Tail-myc, Tbx6-myc, or both proteins. Embryos were injected with RNA at the one-to two-cell stage and analyzed at 80% epiboly for MyoD expression by in situ hybridization. Only embryos with dorsal expression of ectopic proteins, detected immunohistochemically, were analyzed.