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. 2003 Jul 18;100(16):9416–9421. doi: 10.1073/pnas.1533500100

Fig. 4.

Fig. 4.

(a) Osteoclastogenesis in bone marrow cell and osteoblast cocultures. TRAP-positive multinucleated osteoclast numbers were counted after 8-day coculture of bone marrow cells and osteoblasts from male ARKO and WT mice in the presence of 10 nM 1α,25(OH)2D3. (b) Pit area resorbed by osteoclasts over an additional 48-h period of coculture on a dentine slice. (c) Osteoclast formation in cultured M-CSF-dependent bone marrow macrophages. (d) Survival rate of isolated osteoclasts formed during coculture of osteoblasts and bone marrow cells. (e) Gene expression of RANKL and G3PDH in cultured primary osteoblasts from male ARKO and WT littermates.