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. 2003 Jul 18;100(16):9422–9427. doi: 10.1073/pnas.1533509100

Fig. 2.

Fig. 2.

Identification of a putative Drosophila U7 snRNA. (A) A scheme for formation of processing complexes on a Drosophila-specific dH3* pre-mRNA (thick line). (B)A Drosophila Kc nuclear extract was incubated in the presence (lane 4) or absence (lane 3) of the WT dH3* pre-mRNA. Bound RNAs were recovered from 90% of the streptavidin beads, separated in an 8% polyacrylamide gel, and detected by silver staining. Lanes 1 and 2 contain RNA precipitated from the Kc nuclear extract by using anti-Sm (αSm) or a nonspecific mAb, respectively. The pre-mRNA substrate annealed to the 2′-O-methyl adapter oligonuclotide remained in the organic phase during phenol extraction. (C) The remaining portion of each processing reaction (10%) was boiled in SDS-loading dye, and bound proteins were analyzed by Western blotting for the presence of dSLBP by using anti-dSLBP Ab (lanes 1 and 2). Lane 3 contains an aliquot of the nuclear extract used for formation of processing complexes. The star indicates a cross-reacting protein present in Drosophila nuclear extracts.