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. 2003 Jul 24;100(16):9434–9439. doi: 10.1073/pnas.1432964100

Fig. 3.

Fig. 3.

Characterization of the Y250F-YMPM and Y250F-YAAA mutants. (A) In vitro lipid kinase assay. Anti-MT immunoprecipitates (IP) were analyzed for their ability to phosphorylate phosphatidylinositol-4,5-bisphosphate. PI3-kinase product phosphatidylinositol-3,4,5-trisphosphate (PI3,4,5P3) is indicated. Y250F-YMPM showed more MT-associated PI3-kinase activity than WT MT. Y250F-YAAA restored a significant amount of PI3-kinase activity compared with Y250F. (B) Y250F-YMPM and Y250F-YAAA alleles activate Akt. Equal amounts of cell lysates were analyzed by Western blotting with an anti-phospho-Akt Ser-473 antibody (Upper) and an anti-Akt antibody (Lower).