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. 2003 Jul 24;100(16):9434–9439. doi: 10.1073/pnas.1432964100

Fig. 5.

Fig. 5.

Y315F-YMPM restores PI3-kinase association with MT but fails to transform BALB/c3T3 cells to the level of WT MT. (A) In vitro lipid kinase assay. Anti-MT immunoprecipitates (IP) were analyzed for their ability to phosphorylate phosphatidylinositol-4,5-bisphosphate. PI3-kinase product phosphatidylinositol-3,4,5-trisphosphate (PI3,4,5P3) is indicated. (B) Y315-YMPM allele activates Akt to the level of WT MT. Equal amounts of cell lysates were analyzed by Western blotting with an anti-phospho-Akt Ser-473 antibody (Upper) and then an anti-Akt antibody (Lower).