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. 2003 Jul 24;100(16):9434–9439. doi: 10.1073/pnas.1432964100

Fig. 6.

Fig. 6.

WT-YMPM shows enhanced PI3-kinase association but similar transformation activity to WT, whereas WT-YAAA shows enhanced transformation activity without affecting PI3-kinase association with MT. (A) WT-YMPM shows enhanced p85 binding to MT. Cell lysates were immunoprecipitated (IP) with an anti-MT antibody. Samples were analyzed by Western blotting with an anti-p85 antibody (Upper) and an anti-MT antibody (Lower). (B) Enhanced transformation by WT-YAAA is independent of Akt activation. Equal amounts of cell lysates were analyzed by Western blotting with an anti-phospho-Akt Ser-473 antibody (Upper) and then an anti-Akt antibody (Lower). (C) Focus formation assay of Y315F add-back mutants. WT-YMPM does not increase BALB/c3T3 cells transformation, whereas WT-YAAA showed enhanced transformation. BALB/c3T3 cells were infected with viral supernatants and cultured with DMEM containing 7% CS for ≈3 weeks. Viral titers of all viruses were similar (data not shown).