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Expression of relevant genes in 3T3-L1 cells transfected with insig-1. □, mouse insig-1 (m) or human insig-1 (h) transfection; ▪, transfection with empty vector (EV). All cells were cultured in 25 mM glucose with Ins/Dex/IBMX. Data are mean ± SEM of the relative amount of mRNA of interest calculated with 36B4 as standard. *, P < 0.05; **, P < 0.01 (n = 3). Pref-1, preadipocyte factor 1; ACC, acetyl CoA carboxylase.
