Skip to main content
. 2003 Jul 28;100(16):9566–9571. doi: 10.1073/pnas.1633579100

Fig. 1.

Fig. 1.

NOS localization in the brain suggests a role for control of neurogenesis. (a and b) Histochemical detection of NADPH-diaphorase (blue staining) and immunochemical detection of BrdUrd (brown staining). Note that there is significantly less diaphorase staining, representing NOS enzyme, in the bracketed areas, which mark the prolific neurogenic regions of the SVZ and RMS. (c and d) Double labeling of nNOS+ (red) and BrdUrd+ (green) cells in the SVZ (c) and RMS (d). Note that bodies of nNOS-expressing neurons lie outside of the SVZ and RMS, whereas processes run along, but rarely cross, the boundaries of the neurogenic BrdUrd+ areas. Brackets indicate areas of lower NOS staining in the SVZ (a and c) and RMS (a and b) as compared with neighboring parenchyma. In all panels the arrows indicate examples of cell bodies of NOS+ neurons, and arrowheads show varicose NOS+ processes that are proximal to dividing cells (c and d). (Insets) Anatomical position of photomicrographs of sagittal rat brain sections. cc, corpus callosum; ctx, cortex; str, striatum. (Scale bars: a, 1 mm; b, 200 μm; c and d, 50 μm.)