Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1981 May;38(2):777–781. doi: 10.1128/jvi.38.2.777-781.1981

Encephalomyocarditis Viral RNA Can Be Translated Under Conditions of Poliovirus-Induced Translation Shutoff In Vivo

Barbara Morgan Detjen 1, George Jen 1, Robert E Thach 1
PMCID: PMC171208  PMID: 6264143

Abstract

Superinfection with poliovirus of HeLa cells already infected with encephalomyocarditis (EMC) virus does not inhibit translation of EMC viral mRNA, whereas residual host translation is completely inhibited. This result indicates that the cap recognition factors inactivated by poliovirus are not required for translation of EMC viral mRNA in vivo, in agreement with previous in vitro experiments. This raises the question of why EMC virus has evolved a capindependent translation mechanism.

Full text

PDF
777

Images in this article

779Image
on p.779
779Image
on p.779

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abreu S. L., Lucas-Lenard J. Cellular protein synthesis shutoff by mengovirus: translation of nonviral and viral mRNA's in extracts from uninfected and infected Ehrlich ascites tumor cells. J Virol. 1976 Apr;18(1):182–194. doi: 10.1128/jvi.18.1.182-194.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baglioni C., Maroney P. A., West D. K. 2'5'Oligo(A) polymerase activity and inhibition of viral RNA synthesis in interferon-treated HeLa cells. Biochemistry. 1979 May 1;18(9):1765–1770. doi: 10.1021/bi00576a020. [DOI] [PubMed] [Google Scholar]
  3. Bonatti S., Sonenberg N., Shatkin A. J., Cancedda R. Restricted initiation of protein synthesis on the potentially polycistronic Sindbis virus 42 S RNA. J Biol Chem. 1980 Dec 10;255(23):11473–11477. [PubMed] [Google Scholar]
  4. Ehrenfeld E., Lund H. Untranslated vesicular stomatitis virus messenger RNA after poliovirus infection. Virology. 1977 Jul 15;80(2):297–308. doi: 10.1016/s0042-6822(77)80006-8. [DOI] [PubMed] [Google Scholar]
  5. Fernández-Puentes C., Carrasco L. Viral infection permeabilizes mammalian cells to protein toxins. Cell. 1980 Jul;20(3):769–775. doi: 10.1016/0092-8674(80)90323-2. [DOI] [PubMed] [Google Scholar]
  6. Golini F., Thach S. S., Birge C. H., Safer B., Merrick W. C., Thach R. E. Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3040–3044. doi: 10.1073/pnas.73.9.3040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorbalenya A. E., Svitkin Y. V., Kazachkov Y. A., Agol V. I. Encephalomyocarditis virus-specific polypeptide p22 is involved in the processing of the viral precursor polypeptides. FEBS Lett. 1979 Dec 1;108(1):1–5. doi: 10.1016/0014-5793(79)81164-3. [DOI] [PubMed] [Google Scholar]
  8. Helentjaris T., Ehrenfeld E., Brown-Luedi M. L., Hershey J. W. Alterations in initiation factor activity from poliovirus-infected HeLa cells. J Biol Chem. 1979 Nov 10;254(21):10973–10978. [PubMed] [Google Scholar]
  9. Hewlett M. J., Rose J. K., Baltimore D. 5'-terminal structure of poliovirus polyribosomal RNA is pUp. Proc Natl Acad Sci U S A. 1976 Feb;73(2):327–330. doi: 10.1073/pnas.73.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jen G., Birge C. H., Thach R. E. Comparison of initiation rates of encephalomyocarditis virus and host protein synthesis in infected cells. J Virol. 1978 Sep;27(3):640–647. doi: 10.1128/jvi.27.3.640-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jen G., Detjen B. M., Thach R. E. Shutoff of HeLa cell protein synthesis by encephalomyocarditis virus and poliovirus: a comparative study. J Virol. 1980 Jul;35(1):150–156. doi: 10.1128/jvi.35.1.150-156.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lawrence C., Thach R. E. Encephalomyocarditis virus infection of mouse plasmacytoma cells. I. Inhibition of cellular protein synthesis. J Virol. 1974 Sep;14(3):598–610. doi: 10.1128/jvi.14.3.598-610.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCormick W., Penman S. Replication of mengovirus in HeLa cells preinfected with nonreplicating poliovirus. J Virol. 1968 Sep;2(9):859–864. doi: 10.1128/jvi.2.9.859-864.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nomoto A., Lee Y. F., Wimmer E. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np. Proc Natl Acad Sci U S A. 1976 Feb;73(2):375–380. doi: 10.1073/pnas.73.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Palmenberg A. C., Pallansch M. A., Rueckert R. R. Protease required for processing picornaviral coat protein resides in the viral replicase gene. J Virol. 1979 Dec;32(3):770–778. doi: 10.1128/jvi.32.3.770-778.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rose J. K., Trachsel H., Leong K., Baltimore D. Inhibition of translation by poliovirus: inactivation of a specific initiation factor. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2732–2736. doi: 10.1073/pnas.75.6.2732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shirman G. A., Maslova S. V., Gavrilovskaya I. N., Agol V. I. Stimulation of restricted reproduction of EMC virus in HeLa cells by non-replicating poliovirus. Virology. 1973 Jan;51(1):1–10. doi: 10.1016/0042-6822(73)90360-7. [DOI] [PubMed] [Google Scholar]
  18. Sonenberg N., Trachsel H., Hecht S., Shatkin A. J. Differential stimulation of capped mRNA translation in vitro by cap binding protein. Nature. 1980 May 29;285(5763):331–333. doi: 10.1038/285331a0. [DOI] [PubMed] [Google Scholar]
  19. Svitkin Y. V., Ginevskaya V. A., Ugarova T. Y., Agol V. I. A cell-free model of the encephalomyocarditis virus-induced inhibition of host cell protein synthesis. Virology. 1978 Jun 1;87(1):199–203. doi: 10.1016/0042-6822(78)90172-1. [DOI] [PubMed] [Google Scholar]
  20. Trachsel H., Sonenberg N., Shatkin A. J., Rose J. K., Leong K., Bergmann J. E., Gordon J., Baltimore D. Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1980 Feb;77(2):770–774. doi: 10.1073/pnas.77.2.770. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES