Table 1.

Summary of the purification protocol of the 1,3-1,4-β-glucanase produced by Rhizopus microsporus var. microsporus.

Steps	Total Protein(mg)	Total Activity(U)	Specific activity(U.mg-1)	Purification (-fold)	Yield(%)
Concentrated crude extract	1.574	0.228	0.197	1	100.000
Sephacryl S-100 eluate	0.235	0.227	0.966	4.915	99.561
SP – Sepharose eluate	0.021	0.262	12.596	55.529	114.912