TABLE I.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| SEY6210 | MATα his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 GAL | Ref. 34 |
| FRY138 | SEY6210; ATG9-YFP::HIS5 S.p. atg1Δ::URA3 | Ref. 27 |
| TYY014 | SEY6210; ATG20-YFP::HIS5 S.p. vps38Δ::LEU2 K.l. atg11Δ::URA3 K.l. | This study |
| YTS135 | SEY6210; ATG9-YFP::HIS5 S.p. atg1Δ::URA3 atg19Δ::LEU2 K.l. | This study |
| YTS136 | SEY6210; ATG9-YFP::HIS5 S.p. atg1Δ::URA3 ape1 ::LEU2 | This study |
| YTS150 | SEY6210; ATG9-YFP::HIS5 S.p. atg1Δ::URA3 atg11Δ::LEU2 K.l. | This study |
| YTS180 | SEY6210; ATG20-YFP::HIS5 S.p. vps38Δ::LEU2 K.l. | This study |
| YTS184 | SEY6210; ATG20-YFP::HIS5 S.p. vps38Δ::LEU2 K.l. atg19Δ::URA3 K.l. | This study |
| YTS185 | SEY6210; ATG20-YFP::HIS5 S.p. vps38Δ::LEU2 K.l. ape1Δ::TRP1 | This study |
| YTS187 | SEY6210; URA3::GTP-ATG8 | This study |
| YTS188 | SEY6210; atg1Δ::HIS5 S.p. GFP-ATG8:: URA3 | This study |
| YTS189 | SEY6210; pep4Δ:: LEU2 GFP-ATG8::URA3 | This study |
| YTS190 | SEY6210; atg19Δ::HIS5 S.p. GFP-ATG8::URA3 | This study |
| YTS191 | SEY6210; ape1Δ::LEU2 GFP-ATG8::URA3 | This study |
| YTS192 | SEY6210; atg11Δ::HIS3 GFP-ATG8::URA3 | This study |
| YTS193 | SEY6210; atg2Δ::HIS5 S.p. [GFP-ATG2 URA3] | This study |
| YTS194 | SEY6210; atg2Δ::HIS5 S.p. atg19 ::LEU2 K.l. [GFP-ATG2 URA3] | This study |
| YTS195 | SEY6210; atg2Δ::HIS5 S.p. ape1 ::LEU2 [GFP-ATG2 URA3] | This study |
| YTS196 | SEY6210; atg2Δ::HIS5 S.p. atg11 ::LEU2 K.l. GFP-ATG2 URA3 | This study |
| YTS197 | SEY6210 [GFP-ATG1 URA3] | This study |
| YTS198 | SEY6210; atg19Δ::HIS5 S.p. [GFP-ATG1 URA3] | This study |
| YTS199 | SEY6210; ape1Δ::LEU2 [GFP-ATG1 URA3] | This study |
| YTS200 | SEY6210; atg11Δ::HIS3 [GFP-ATG1 URA3] | This study |
| YTS201 | SEY6210; atg1Δ::HIS5 S.p. URA3::GFP-ATG8 [atg1tsTRP1] | This study |
| YTS202 | SEY6210; atg1Δ::HIS5 S.p. atg19 ::LEU2 K.l. URA3::GFP-ATG8 [atg1tsTRP1] | This study |
| YTS203 | SEY6210; atg1Δ::HIS5 S.p. ape1 ::LEU2 URA3::GFP-ATG8 [atg1tsTRP1] | This study |
| YTS204 | SEY6210; atg1Δ::HIS5 S.p. atg11Δ::LEU2 K.l. URA3::GFP-ATG8 [atg1tsTRP1] | This study |