Absorption (top, solid), fluorescence (top, dashed), and stimulated Raman spectra (bottom) of rhodamine 6G, chlorophyll a, and DTTCI showing that stimulated Raman spectra are obtained easily from molecules with high fluorescence backgrounds. The rhodamine 6G (1.3 mM in methanol) and chlorophyll a (0.6 mM in ethanol) spectra were each collected in 11 s, while the DTTCI (2 μM in methanol) spectrum was collected in 2.2 s. In each Raman spectrum the solvent peaks have been subtracted; the asterisk (*) indicates an artifact from this subtraction. Experimental conditions: Raman pump =793 nm, 1.5 μJ/pulse, 800 fs; pathlength =1 cm.