Ethanol in honey bee hemolymph decreases as a function of time post ethanol ingestion. The concentration of the ethanol in the hemolymph of subjects that consumed 1.0 M sucrose solution containing 5, 10, 25, or 50% ethanol was measured at different times (0.5, 6, 12, 24 or 48 h) post-ingestion. Ethanol levels were significantly lower for the 24 and 48 h time points when compared with the 0.5 h time point for all treatments. All treatments, except the 10 and 25%, had significantly different ethanol concentrations (P ≤ 0.001). Each point represents the mean +/− SEM. At 0.5 hr: N5% = 7, N10% = 8, N25% = 7, N50% = 8, Ncontrol = 7; at 6 hr: N5% = 6, N10% = 5, N25% = 6, N50% = 6, Ncontrol = 6; at 12 hr: N5% = 5, N10% = 6, N25% = 5, N50% = 5, Ncontrol = 5; at 24 hr: N5% = 7, N10% = 6, N25% = 7, N50% = 7, Ncontrol = 6; and at 48 hr: N5% = 7, N10% = 5, N25% = 7, N50% = 5, Ncontrol = 8.