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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2006 Oct 5;81(3-4):136–149. doi: 10.1016/j.prostaglandins.2006.09.001

Figure 1.

Figure 1

Dot blot screening for anti-oleamide antibodies. Equivalent amounts (3-5 μg) of native bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH) and transferrin (TF) proteins (top row), or their respective oleamide conjugates (bottom row) were spotted to Nytran® membrane. The blot was blocked and then incubated with antibody raised against oleamide conjugated to KLH (ODA-1 diluted 1:4,000). Following extensive washing, bound primary antibody was visualized by enhanced chemiluminesence using a goat anti-rabbit secondary antibody coupled to horseradish peroxidase.