Fig 2.

 Hap46/BAG-1M forms complexes with various transcription factors. Transcription factors, generated as radiolabeled proteins by in vitro transcription/translation from recombinant constructs, were incubated in the presence of Hsc70, contained in the reticulocyte lysate, with Sepharose matrices to which either Hap46/BAG-1M (lane 1) or deletion variant Hap46/BAG-1MΔC47 (lane 2) were attached. After extensive washing, retained proteins were eluted and analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. GR, glucocorticoid receptor; ER, estrogen receptor. (Modified from Niyaz 2003; Niyaz et al 2003)