Fig 3.

 Stimulation of transcription by Hap46/BAG-1M and Hap50/ BAG-1L. (A) Human DU145 cells were transfected with an expression vector encoding Hap46/BAG1M (lanes 3, 4) or not (lanes 1, 2). As indicated, cells were submitted to heat shock for 2 hours at 42°C. Newly synthesized poly(A)⁺ RNA was analyzed by gel electrophoresis and autoradiography (upper panel), the intensity of blackening giving a measure for the stimulation of transcription. The positions of 28S and 18S rRNAs are shown along the margin. The same filters were subsequently used for Northern hybridization with labeled cDNAs for Hsp70 and Hsp40 (lower panels) (from Zeiner et al 1999). (B) Human HeLa cells were transfected with an expression vector encoding Hap50/BAG-1L (lower panel) or not (upper panel) and used for nuclear runoff transcription assays. Labeled RNAs were probed with dot-blotted cDNAs for GR (= glucocorticoid receptor), ER (= estrogen receptor), c-Jun, or c-Fos (from Niyaz et al 2001)